Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies

Brazilian Journal of Microbiology

Andrea Von Groll²  Anandi Martin²  Françoise Portaels²  Pedro Eduardo Almeida Da Silva¹  Juan Carlos Palomino² 

[1] ,Institute of Tropical Medicine Antwerp Mycobacteriology Unit Antwerp,Belgium

关键词: Growth kinetics;    Mycobacterium tuberculosis;    Resazurin;    Fitness;   

DOI  :  10.1590/S1517-83822010000200006

来源: SciELO

【 摘 要 】

We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate.

【 授权许可】

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