Growth kinetics of Mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies

Revista de microbiologia

von Groll, Andrea¹  Martin, Anandi² 

[1] Institute of Tropical Medicine Antwerp, Antwerp, Belgium;Universidade Federal do Rio Grande, Rio Grande, Brasil

关键词: Growth kinetics;    Mycobacterium tuberculosis;    Resazurin;    Fitness.;   

DOI  :  10.1590/S1517-83822010000200006

学科分类：农业科学（综合）

来源: Sociedade Brasileira de Microbiologia / Brazilian Society for Microbiology

【 摘 要 】

We standardized a method to evaluate the growth kinetics of Mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. Growth curves and the rate of growth of twenty-one M. tuberculosis clinical isolates were determined. The method showed technical simplicity and is inexpensive to assess the fitness of each isolate.

【 授权许可】

CC BY-NC