Phylogenetic analysis on the soil bacteria distributed in karst forest

Brazilian Journal of Microbiology

Junpei Zhou¹  Ying Huang¹  Minghe Mo¹ 

[1] ,Yunnan University Laboratory for Conservation and Utilization of Bio-resources Kunming P. R. ,China

关键词: karst forest;    bacteria;    16S rRNA gene;   

DOI  :  10.1590/S1517-83822009000400013

来源: SciELO

【 摘 要 】

Phylogenetic composition of bacterial community in soil of a karst forest was analyzed by culture-independent molecular approach. The bacterial 16S rRNA gene was amplified directly from soil DNA and cloned to generate a library. After screening the clone library by RFLP, 16S rRNA genes of representative clones were sequenced and the bacterial community was analyzed phylogenetically. The 16S rRNA gene inserts of 190 clones randomly selected were analyzed by RFLP and generated 126 different RFLP types. After sequencing, 126 non-chimeric sequences were obtained, generating 113 phylotypes. Phylogenetic analysis revealed that the bacteria distributed in soil of the karst forest included the members assigning into Proteobacteria, Acidobacteria, Planctomycetes, Chloroflexi (Green nonsulfur bacteria), Bacteroidetes, Verrucomicrobia, Nitrospirae, Actinobacteria (High G+C Gram-positive bacteria), Firmicutes (Low G+C Gram-positive bacteria) and candidate divisions (including the SPAM and GN08).

【 授权许可】

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