Novel agents for enzymatic and fungal hydrolysis of stevioside

Brazilian Journal of Microbiology

H.m.s. Milagre¹  L.r. Martins¹  J.a. Takahashi¹ 

[1],Universidade Federal de Minas Gerais Departamento de Química Belo Horizonte MG ,Brasil

关键词: stevioside;    hydrolysis;    Aspergillus niger;    lipase;    pancreatin;    esteviosídeo;    hidrólise;    Aspergillus niger;    lipase;    pancreatina;   

DOI  :  10.1590/S1517-83822009000200029

来源: SciELO

【 摘 要 】

A comparative study on the potential of some biological agents to perform the hydrolysis of stevioside was carried out, aiming at establishing an alternative methodology to achieve the aglycon steviol or its rearranged derivative isosteviol, in high yields to be used in the preparation of novel bioactive compounds. Hydrolysis reactions were performed by using filamentous fungi (Aspergillus niger, Rhizopus stolonifer and Rhizopus arrhizus), a yeast (Saccharomyces cerevisiae)andenzymes (pancreatin and lipases PL250 and VFL 8000). Pancreatin showed the best hydrolytic activity, furnishing isosteviol at 93.9% of yield, at pH 4.0, using toluene as a co-solvent. Steviol was produced using both pancreatin at pH 7.0 (20.2% yield) and A. niger at pH 7 (20.8% yield).

【 授权许可】

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