Lipase Immobilization on Silica Xerogel Treated with Protic Ionic Liquid and its Application in Biodiesel Production from Different Oils

International Journal of Molecular Sciences

Silvana Mattedi¹  Lisianedos S. Freitas²  CleideM. F. Soares³  NayáraB. Carvalho³  BrunaT. Vidal³  AndersonS. Barbosa³  MatheusM. Pereira³  ÁlvaroS. Lima³ 

[1]Department of Chemical Engineering, Federal University of Bahia, Rua Prof. Aristides Novis, 02, Federação, Salvador 40210-630, Bahia, Brazil

[2]Department of Chemistry, Federal University of Sergipe, Av. Marechal Rondon, s/n, Jd. Rosa Elze, São Cristóvão 49100-000, Sergipe, Brazil

[3]Institute of Technology and Research, Avenida Murilo Dantas 300, Aracaju 49032-490, Sergipe, Brazil

关键词: treated silica xerogel support;    protic ionic liquid;    lipase;    immobilization;    hydrolysis;    transesterification;   

DOI  :  10.3390/ijms19071829

来源: DOAJ

【 摘 要 】

Treated silica xerogel with protic ionic liquid (PIL) and bifunctional agents (glutaraldehyde and epichlorohydrin) is a novel support strategy used in the effective immobilization of lipase from Burkholderia cepacia (LBC) by covalent binding. As biocatalysts with the highest activity recovery yields, LBC immobilized by covalent binding with epichlorohydrin without (203%) and with PIL (250%), was assessed by the following the hydrolysis reaction of olive oil and characterized biochemically (Michaelis–Menten constant, optimum pH and temperature, and operational stability). Further, the potential transesterification activity for three substrates: sunflower, soybean, and colza oils, was also determined, achieving a conversion of ethyl esters between 70 and 98%. The supports and the immobilized lipase systems were characterized using Fourier transform infrared spectra (FTIR), scanning electron microscopy (SEM), elemental analysis, and thermogravimetric (TG) analysis.

【 授权许可】

Unknown