Production of 6-pentyl-α-pyrone by trichoderma harzianum in solid-state fermentation

Brazilian Journal of Microbiology

Aline De Souza Ramos²  Sorele Batista Fiaux¹  Selma Gomes Ferreira Leite¹ 

[1] ,Fundação Oswaldo Cruz Farmanguinhos Rio de Janeiro RJ ,Brasil

关键词: 6-pentyl-α-pyrone;    Trichoderma harzianum;    solid-state fermentation;    experimental design;    6-pentil-α-pirona;    Trichoderma harzianum;    fermentação em estado sólido;    planejamento experimental;   

DOI  :  10.1590/S1517-83822008000400022

来源: SciELO

【 摘 要 】

Many Trichoderma species are able to produce 6-pentyl-α-pyrone (6-PP), a lactone with coconut-like aroma. In the present work, several culture parameters were studied to enhance the production of 6-PP by Trichoderma harzianum 4040 in solid-state fermentation. Green coir powder added to a nutrient solution was used as support material for fermentation. A Plackett-Burman screening technique was applied, followed by a fractionary factorial design. The best culture conditions within the experimental domain studied were (100 g support)-1: sucrose, 3 g; NaNO3, 0.24 g; (NH4)2SO4, 0.18 g; KH2PO4, 0.1 g; inoculum concentration, 2.2 x 10(6) spores; moisture level, 55%. The temperature established was 28ºC. The fermentation under the selected conditions led to a 6-PP production six times higher (5.0 mg/g dry matter) than the initial one (0.8 mg/g dry matter) after seven days of cultivation.

【 授权许可】

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