Extended-spectrum β-lactamases producing Klebsiella pneumoniae isolated in two hospitals in Goiânia/Brazil: detection, prevalence, antimicrobial susceptibility and molecular typing

Brazilian Journal of Microbiology

Daniella Fabíola Dos Santos²  Fabiana Cristina Pimenta¹  Rodrigo Alves²  Edlaine Rodrigues Montalvão²  Daniela Braz Dos Santos²  José Rodrigues Do Carmo Filho² 

[1] ,Universidade Católica de Goiás GO ,Brasil

关键词: Extended-spectrum β-lactamases (ESBL);    Klebsiella pneumoniae;    β-lactamases;    bacterial resistance;    Extended-spectrum β-lactamases (ESBL);    Klebsiella pneumoniae;    β-lactamases;    resistência bacteriana;   

DOI  :  10.1590/S1517-83822008000400002

来源: SciELO

【 摘 要 】

This study was developed to evaluate the prevalence of extended-spectrum β-lactamases (ESBL) producing Klebsiella pneumoniae in two hospitals (A and B) in Goiânia, GO, Brazil. The antimicrobial susceptibility of the isolates was determined using the MicroScan WalkAway (Dade Behring, USA). Tests to evaluate the genetic correlation between the isolates were also performed. For the ESBL phenotypic test, the Double-disk diffusion (DD) method was used. The strainsisolated in Hospital B were submitted to DNA analysis by pulsed-field gel electrophoresis (PFGE). The study showed high prevalence of ESBL-producing K. pneumoniae (25% in hospital A and 66.7% in hospital B), with high rates of antimicrobial resistance. The most active compound was imipenem (100% susceptibility in vitro). The PFGE test showed similiarity in five strains and variability in six strains.The high prevalence of ESBL-producing Klebsiella may be due to individual selection and to dissemination of a common strain.

【 授权许可】

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