Isolation and characterization of Leptospira interrogans from pigs slaughtered in São Paulo State, Brazil

Brazilian Journal of Microbiology

Fabiana Miraglia²  Andréa Mike Moreno²  Cleise Ribeiro Gomes²  Renata Paixão²  Esequiel Liuson¹  Zenaide Maria Morais²  Paulo Maiorka¹  Fabiana Kömmling Seixas¹  Odir Antonio Dellagostin¹  Silvio Arruda Vasconcellos² 

[1] ,Universidade de São Paulo Faculdade de Medicina Veterinária e Zootecnia Departamento de Medicina Veterinária Preventiva e Saúde AnimalSão Paulo SP ,Brasil

关键词: Pomona;    Swine;    VNTR;    Culture;    PCR;    Genital Tract;    Pomona;    Suínos;    VNTR;    Isolamento;    PCR;    Trato Genital;   

DOI  :  10.1590/S1517-83822008000300017

来源: SciELO

【 摘 要 】

With the aim of isolating Leptospira spp., blood serum, kidney, liver and genital tract of 137 female swine (40 sows and 97 gilts) and also urine samples from 22 sows were collected in a slaughterhouse in the State of São Paulo, from April 2003 to August 2004. Four isolates were obtained from animals that presented microagglutination test (MAT) titers > 100 for the serovar Pomona and one was obtained from an animal negative by MAT in which Leptospira was isolated from the liver and reproductive tract. The presence of leptospiral DNA was investigated by PCR, and positive results were found in kidneys of 11 females, liver of two, genital tract of two and urine of one of them. Nephrosis, interstitial multifocal nephritis, moderate to severe changing, hyalines cylinders and hemorrhagic focuses, hepatic and uterine horns congestion were histological lesions observed in higher frequency in animals positive for leptospira. The silver impregnation (Warthin Starry) confirmed the presence of spirochetes in renal tubules of four females with positive leptospira cultures from kidneys. The serogroup of the five isolates was identified as Pomona by cross agglutination with reference polyclonal antibodies. Molecular characterization of the isolates was carried out by variable-number tandem-repeats analysis. All the isolates revealed a pattern distinct from the L. interrogans Pomona type strain, but identical to a previously identified pattern from strains isolated in Argentina belonging to serovar Pomona.

【 授权许可】

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