Molecular genetic differentiation of avian Escherichia coli by RAPD-PCR

Brazilian Journal of Microbiology

Taghi Zahraei Salehi²  Seyed Ahmad Madani¹  Vahid Karimi¹  Fatemeh Arab Khazaeli¹ 

[1] ,University of Tehran Faculty of Veterinary Medicine Department of MicrobiologyTehran,Iran

关键词: Escherichia coli;    RAPD-PCR;    Molecular differentiation;    Iran;    Escherichia coli;    RAPD-PCR;    diferenciação molecular;    Irã;   

DOI  :  10.1590/S1517-83822008000300015

来源: SciELO

【 摘 要 】

Escherichia coli is one of the most important bacterial avian pathogens and a common inhabitant of the gastrointestinal tract of animals. Most pathogenic E. coli can not be differentiated biochemically or by classic microbiologic methods. Molecular typing methods, particularly PCR, facilitated epidemiological and ecological studies of bacteria. Here we describe the application of a random amplified polymorphic DNA- polymerase chain reaction (RAPD-PCR) for molecular genetic differentiation of E. coli isolates in Iran. In this study 58 E. coli isolates including 4 standard strains, 3 food originated isolates, 33 avian isolates, 8 isolates form diarrheic calves and 10 isolates from unweaned diarrheic lambs were analyzed by RAPD-PCR using primer 1247(5/-AAG AGC CCG T-3/). The RAPD analysis showed that these isolates could be grouped into 33 RAPD types and avian isolates were discriminated into 29 genotypes. It was shown that the primer could not differentiate E. coli isolated from lambs. Discriminatory index for entire isolates was 0.912 and for avian isolates was 0.990. We concluded that RAPD-PCR can be used as a method for molecular differentiation of E. coli isolates.

【 授权许可】

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