Lectin-binding properties of Aeromonas caviae strains

Brazilian Journal of Microbiology

Cláudio M. Rocha-de-souza¹  Raphael Hirata-jr¹  Ana L. Mattos-guaraldi¹  Angela C. Freitas-almeida¹  Arnaldo F. B. Andrade¹ 

[1] ,Universidade do Estado do Rio de Janeiro Faculdade de Ciências Médicas Rio de Janeiro RJ ,Brasil

关键词: Aeromonas caviae;    Lectin;    Carbohydrate;    Cell surface;    Aeromonas caviae;    Lectina;    Carbohidrato;    Superficie celular;   

DOI  :  10.1590/S1517-83822008000200003

来源: SciELO

【 摘 要 】

The cell surface carbohydrates of four strains of Aeromonas caviae were analyzed by agglutination and lectin-binding assays employing twenty highly purified lectins encompassing all sugar specificities. With the exception of L-fucose and sialic acid, the sugar residues were detected in A. caviae strains. A marked difference, however, in the pattern of cell surface carbohydrates in different A. caviae isolates was observed. Specific receptors for Tritricum vulgaris (WGA), Lycopersicon esculentum (LEL) and Solanum tuberosum (STA) (D-GlcNAc-binding lectins) were found only in ATCC 15468 strain, whereas Euonymus europaeus (EEL, D-Gal-binding lectin) sites were present exclusively in AeQ32 strain, those for Helix pomatia (HPA, D-GalNAc-binding lectin) in AeC398 and AeV11 strains, and for Canavalia ensiformes (Con A, D-Man-binding lectin) in ATCC 15468, AeC398, AeQ32 and AeV11 strains, after bacterial growing at 37ºC. On the other hand, specific receptors for WGA and EEL were completely abrogated growing the bacteria at 22ºC. Binding studies with 125I- labeled lectins from WGA, EEL and Con A were performed. These assays essentially confirmed the selectivity, demonstrated in the agglutination assays of these lectins for the A. caviae strains.

【 授权许可】

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