The role of carbohydrates in the radioimmunoassay of human low‐molecular‐mass kininogen

FEBS Letters

Turpeinen, Ursula¹  Kärkkäinen, Tytti¹ 

[1] Department of Biochemistry, University of Helsinki, Unioninkatu 35, 00170 Helsinki, Finland

关键词: Carbohydrate;    Glycoprotein;    Kininogen;    Radioimmunoassay;    Lectin;    Exoglycosidase;   

DOI  :  10.1016/0014-5793(85)81010-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The immunoreactivity of human low-molecular-mass kininogen from Cohn plasma fraction IV was investigated after deglycosylations and carbohydrate modifications by radioimmunoassay using the conformation-specific antiserum. Removal of all sialic acids, 44% of amino sugars and 63% of neutral sugars did not alter the immunoreactivity of the protein but the periodate-treated concanavalin A fractions showed strikingly diminished immunoreactivity. A conformational change could account for the observed effect of periodate on the decreased reactivity of the protein in radioimmunoassay. Externally added carbohydrates had no effect on immunoreactivity. The results suggest that the carbohydrate part of kininogen is not involved in the immunoreactivity although it accounts for the observed lectin-binding heterogeneity.

【 授权许可】

Unknown