期刊论文详细信息
FEBS Letters
Temperature‐induced ultraviolet difference absorption spectrometry for determination of enthalpy changes
Loontiens, Frank G.1  Dhollander, Gaby1 
[1] Laboratorium voor Biochemie, Faculteit Wetenschappen, Rijksuniversiteit Gent, Ledeganckstraat 35, B-9000 Gent, Belgium
关键词: Lectin;    Carbohydrate;    Binding enthalpy;    Difference absorption spectrometry;    Con A;    concanavalin A;    ECA;    Erythrina cristagalli agglutinin;    SBA;    soybean agglutinin;    MeUmb-;    4-methylumbelliferyl-;    all sugars mentioned;    are of the D-pyranoside configuration;   
DOI  :  10.1016/0014-5793(84)80745-0
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Raising the temperature in a single mixture of a lectin and a chromophoric glycoside allows determination of the binding enthalpy. This is made possible by continuously monitoring the displacement of the complex from its equilibrium concentration with a sensitive difference absorption spectrophotometer. The method is illustrated with the following lectins: concanavalin A, soybean agglutinin, peanut agglutinin and Erythrina cristagalli agglutinin. The ligands are 4-methylumbelliferyl glycosides. The binding enthalpies found range from −60 kJ · mol−1 for the Galß1 → 3GalNAc-ßglycoside and peanut agglutinin to −30 kJ · mol−1 for a monosaccharide glycoside and the other lectins.

【 授权许可】

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