Temperature‐induced ultraviolet difference absorption spectrometry for determination of enthalpy changes

FEBS Letters

Loontiens, Frank G.¹  Dhollander, Gaby¹ 

[1] Laboratorium voor Biochemie, Faculteit Wetenschappen, Rijksuniversiteit Gent, Ledeganckstraat 35, B-9000 Gent, Belgium

关键词: Lectin;    Carbohydrate;    Binding enthalpy;    Difference absorption spectrometry;    Con A;    concanavalin A;    ECA;    Erythrina cristagalli agglutinin;    SBA;    soybean agglutinin;    MeUmb-;    4-methylumbelliferyl-;    all sugars mentioned;    are of the D-pyranoside configuration;   

DOI  :  10.1016/0014-5793(84)80745-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Raising the temperature in a single mixture of a lectin and a chromophoric glycoside allows determination of the binding enthalpy. This is made possible by continuously monitoring the displacement of the complex from its equilibrium concentration with a sensitive difference absorption spectrophotometer. The method is illustrated with the following lectins: concanavalin A, soybean agglutinin, peanut agglutinin and Erythrina cristagalli agglutinin. The ligands are 4-methylumbelliferyl glycosides. The binding enthalpies found range from −60 kJ · mol⁻¹ for the Galß1 → 3GalNAc-ßglycoside and peanut agglutinin to −30 kJ · mol⁻¹ for a monosaccharide glycoside and the other lectins.

【 授权许可】

Unknown