Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods

Brazilian Journal of Microbiology

Bruna C. Gomes¹  Carolina T. Esteves²  Izabel C.v. Palazzo²  Ana Lúcia C. Darini²  Bernadette D.g.m. Franco¹  Elaine C.p. De Martinis² 

[1] ,Universidade de São Paulo Faculdade de Ciências Farmacêuticas São Paulo SP ,Brasil

关键词: Enterococcus spp.;    identification;    API 20 STREP;    PCR multiplex;    foods;    Enterococcus spp.;    identificação;    API 20 STREP;    PCR multiplex;    alimentos;   

DOI  :  10.1590/S1517-83822007000400007

来源: SciELO

【 摘 要 】

We evaluated the suitability of API 20 STREP and multiplex PCR to speciate 52 Enterococcus spp. obtained from Brazilian foods. A high percentage of isolates (78.9%) presented discrepant results between evaluated tests. Similar results were obtained for six E. faecalis and five E. faecium. The PCR multiplex was more effective than API 20 STREP for complete identification of the isolates.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License