Formation of biofilms by Staphylococcus aureus on stainless steel and glass surfaces and its resistance to some selected chemical sanitizers

Brazilian Journal of Microbiology

Simone Cristina Marques¹  Jaíne Das Graças Oliveira Silva Rezende¹  Lizandra Aparecida De Freitas Alves¹  Belami Cássia Silva¹  Eduardo Alves¹  Luiz Ronaldo De Abreu¹  Roberta Hilsdorf Piccoli¹ 

[1] ,Universidade Federal de Lavras Departamento de Ciência dos Alimentos Lavras MG ,Brasil

关键词: Biofilm;    stainless steel;    glass;    Staphylococcus aureus;    sanitizers;    scanning electron microscopy;    Biofilme;    aço inoxidável;    vidro;    Staphylococcus aureus sanificantes;    microscopia eletrônica de varredura;   

DOI  :  10.1590/S1517-83822007000300029

来源: SciELO

【 摘 要 】

The objectives of this work were to verify the capability of Staphylococcus aureus of forming bio-film on stainless steel and glass surfaces; to evaluate the efficiency of sodium dichloroisocyanurate, hydrogen peroxide and peracetic acid in inactivating Staphylococcus aureus cells adhered onto these surfaces; and to visualize biofilm development by scanning electron microscopy before and after sanitizer treatment. The surfaces studied consisted of 10x20mm chips immersed in Petri dishes containing BHI broth inoculated with S. aureus ATCC 25923. Biofilm formation was observed after 15-day incubation, when the cells were removed using the swab technique, followed by Baird Parker agar plating. Also, the efficiency of the chemical sanitizers on the chip surfaces was tested and the non-removed cells were counted on the Baird-Parker agar. After biofilm formation and use of sanitizers, the chips were respectively observed by scanning electronic microscopy following a pre-existing protocol. The obtained results showed biofilm formation on both surfaces, with bacterial count in the order of 10(7) CFU/cm² on and 10(8) CFU/cm² on stainless steel and glass surfaces, respectively. Peracetic acid was the most efficient in removing adhered cells, presenting 5.26 and 4.5 decimal reduction for adhered cells on stainless steel and glass surfaces, respectively.

【 授权许可】

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