Interference of tuberculosis on the performance of ELISAs used in the diagnosis of paratuberculosis in cattle

Brazilian Journal of Microbiology

Walter Lilenbaum²  Rachel Ferreira²  Carla Dray Marassi¹  Paula Ristow¹  Walter Martins Roland Oelemann¹  Leila De Souza Fonseca¹ 

[1] ,Universidade Federal Fluminense Laboratório de Bacteriologia Veterinária Niterói RJ ,Brasil

关键词: Paratuberculosis;    Tuberculosis;    Mycobacterium bovis;    Mycobacterium avium paratuberculosis;    paratuberculose;    tuberculose;    Mycobacterium bovis;    Mycobacterium avium paratuberculosis;   

DOI  :  10.1590/S1517-83822007000300016

来源: SciELO

【 摘 要 】

Forty-four cows from five herds infected with tuberculosis (TB) and without paratuberculosis (PTB), and 21 cows from a herd without either infection were studied. The cattle presented concordant results in both the skin test and gamma-interferon assay for TB and two commercial ELISAs for PTB. Animals were divided according to TB test results into Group A with 28 TB-infected animals, Group B with 16 TB-negative animals from infected herds, and Group C with 21 TB-negative cows from a tuberculosis-free herd (which were used as controls). Twenty of 28 animals from Group A (71.4%), 6/16 from Group B (37.5%) and none from Group C were reactive to PTB ELISAs, suggesting that these commercial kits were unable to distinguish between PTB and TB. It is proposed that natural occurring TB strongly interferes in the diagnosis of PTB and that commercial ELISAs cannot be considered reliable tools in the diagnosis of paratuberculosis in tuberculosis-infected herds.

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