Screening of filamentous fungi for production of enzymes of biotechnological interest

Brazilian Journal of Microbiology

Luis Henrique S. Guimarães²  Simone C. Peixoto-nogueira¹  Michele Michelin²  Ana Carolina S. Rizzatti²  Valéria C. Sandrim¹  Fabiana F Zanoelo²  Ana Carla M.m. Aquino²  Altino B. Junior¹  Maria De Lourdes T. M. Polizeli² 

[1] ,USP Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto Departamento de Biologia

关键词: phosphatase;    xylanase;    glucose oxidase;    pectinase;    amylase;    Rhizopus microsporus var. rhizopodiformis;    fungi;    fosfatase;    xilanase;    glicose oxidase;    pectinase;    amilase;    Rhizopus microsporus var. rhizopodiformis;    fungos;   

DOI  :  10.1590/S1517-83822006000400014

来源: SciELO

【 摘 要 】

Many enzymes produced by fungi have relevant biotechnological applications in several industrial areas. The purpose of this study was to collect and isolate filamentous fungi from soil and humus, plants and sugar cane bagasse of different regions of the São Paulo state. Forty isolates were examined for their ability to produce xylanase, glucose-oxidase, alkaline phosphatase, acid phosphatase, phytase, pectinase and amylase. Among these, twenty three isolates exhibited enzymatic potential. The xylanases produced by two of these isolates (Aspergillus caespitosus and A. phoenicis) showed good potential for pulp bleaching. Among seventeen isolates, at least three produced high levels of glucose-oxidase, being Rhizopus stolonifer and A. versicolor the best producer strains. A. caespitosus, Mucor rouxii, and nine others still not identified were the best producers of phosphatases in submerged fermentation. Pectinase was best produced by IF II and C-8 belong R. stolonifer. Significant levels of amylase were produced by Paecilomyces variotii and A. phoenicis. A remarkable enzyme producer was Rhizopus microsporus var. rhizopodiformis that produced high levels of amylase, alkaline and acid phosphatases, and pectinase. Some morphological structures of this fungus were illustrated using light microscopy (LM) and scanning electron microscopy (SEM). This study contributes to catalogue soil fungi isolated in the state of São Paulo, and provides additional information to support future research about the industrial potential of these microorganisms that may produce enzymes and, eventually, also secondary metabolites with anti-microbial or anti-parasitic activities.

【 授权许可】

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