Enzyme activities of the marine-derived fungus Alternaria alternata cultivated on selected agricultural wastes

Journal of Applied Biological Sciences

A. Mostafa FATEN¹  A. Abd EL ATY ABEER¹ 

关键词: Marine fungi;    lignocellulolytic enzymes;    cellulase;    xylanase;    pectinase;    laccase;    agricultural wastes;    A. alternata.;   

DOI  :  

学科分类：生物科学（综合）

来源: Nobel Bilim ve Arasirma Merkezi/Nobel Science and Research Cente

【 摘 要 】

Studieswerecarriedoutontheenzymeactivitiesofthemarine-derivedfungusAlternariaalternatacultivatedinvariousagro-wastes.Thefungusproducedcellulase,xylanase,pectinaseandlaccasebybothsolid-statfermentation(SSF)andsubmergedfermentation(SMF)techniquesondifferentagriculturalwastes.Thelevelofcellulase,xylanase,pectinaseandlaccaseproductionbySSF(9.68,24.84,52.5and1126.62U/gsolidsubstrate,respectively)onwheatbranafter10daysofincubation,whichconsideredhigherthanthatobtainedbySMF(2.61,16.31,35.28and531.94U/gsolidsubstrate,respectively).Thehighestextractionyieldofcellulaseandxylanasewasachievedwithdistilledwaterwhilethehighestpectinaseandlaccaseextractionwasobtainedwithacetatebuffer(0.05M,pH5).Therefore,thehighenzymeproductionalongwiththeverylowcostofthesubstrate,showedthesuitabilityofthesystemA.alternata-SSFforindustrialpurposes,especiallyfortheuseoftheseenzymesinbiotechnologicalprocessesinhighsaltconcentrationconditions.

【 授权许可】

Unknown