Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat

Brazilian Journal of Microbiology

Andréa Dos Santos Schneid²  Kelly Lameiro Rodrigues²  Davi Chemello¹  Eduardo Cesar Tondo¹  Marco Antônio Zacchia Ayub¹  José Antonio Guimarães Aleixo² 

[1] ,Universidade Federal de Pelotas Centro de Biotecnologia Pelotas RS ,Brasil

关键词: Salmonella;    monoclonal antibody;    policlonal antibody;    ELISA;    Salmonella;    anticorpo monoclonal;    anticorpo policlonal;    ELISA;   

DOI  :  10.1590/S1517-83822006000300027

来源: SciELO

【 摘 要 】

In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonellaenterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of 82% and 98% respectively. The serotyping of the isolates revealed 31 Salmonella enterica serovar Enteritidis, 2 Salmonella enterica serovar Heidelberg, 1 Salmonella enterica serovar Choleraesuis and 1 Salmonella enterica sorovar 6,7:-:-.

【 授权许可】

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