Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA

Toxins

Alexandra H. Heussner¹  Simon Ausländer¹ 

[1] Human and Environmental Toxicology, University of Konstanz, PO-Box 918, 78457 Konstanz, Germany;

关键词: mycotoxin;    ochratoxin B;    monoclonal antibody;    ELISA;    western analysis;   

DOI  :  10.3390/toxins2061582

来源: mdpi

【 摘 要 】

A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 µg/L (8 nM), a limit of quantification (LOQ) of 3.7 µg/L (10 nM), and a 50% inhibitory concentration (IC₅₀) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.

【 授权许可】

CC BY   
© 2010 by the authors; licensee MDPI, Basel, Switzerland