Development of an immunochromatographic test strip for the detection of ochratoxin A in red wine

Food and Agricultural Immunology

Hua Kuang¹  Shanshan Song¹  Steven Suryoprabowo¹  Liqiang Liu¹  Liguang Xu¹ 

[1] State Key Lab of Food Science and Technology, Jiangnan University, Wuxi, JiangSu, People’s Republic of Chin;Collaborative Innovation center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, JiangSu, People’s Republic of Chin;

关键词: Immunochromatographic assay;    monoclonal antibody;    mycotoxin;    ochratoxin A;    strip test;   

DOI  :  10.1080/09540105.2017.1401043

来源: publisher

【 摘 要 】

Humans and animals are exposed to mycotoxins naturally present in foods. Ochratoxin A (OTA), one of the most abundant and toxic mycotoxins, is ubiquitous in nature and harmful to humans and animals. In this study, we developed a rapid, simple, specific, and sensitive lateral-flow immunochromatographic assay for detection of OTA in 0.01 M phosphate-buffered saline solution (PBS) (pH 7.4) and in red wine. We produced sensitive and selective monoclonal antibody against OTA. Under optimized conditions, the cut-off limit of the test strip was 10 ng/mL OTA in 0.01 M PBS (pH 7.4) and in red wine. The results were obtained within 10 min. Therefore, our developed method is useful for the detection of OTA in red wine.

【 授权许可】

CC BY