Rapid detection of zearalenone and its metabolite in corn flour with the immunochromatographic test strip

Food and Agricultural Immunology

Kai Hao¹  Hua Kuang²  Shanshan Song²  Steven Suryoprabowo²  Liqiang Liu² 

[1] School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, People’s Republic of Chin;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of Chin;International Joint Research Laboratory for Biointerface and Biodetection, School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of Chin;

关键词: Immunochromatographic assay;    monoclonal antibody;    mycotoxin;    strip test;    zearalenone;   

DOI  :  10.1080/09540105.2017.1406461

来源: publisher

【 摘 要 】

Zearalenone (ZEN) is a mycotoxin produced by fungal species which are widespread in nature and commonly contaminate many cereal grains, such as wheat, maize, corn, barley, and other cereal grains. An immunochromatographic (ICA) test strip was developed for the rapid simultaneous detection of ZEN and its metabolite in corn flour samples. For the ICA test, antigen (ZEN-BSA) and goat anti-mouse IgG were, respectively, drawn on nitrocellulose membrane as control line and test line. Under the optimized condition, the cut-off limits of test strips for ZEN, α-ZAL, and β-ZAL were found to be 0.5 ng/mL in 0.01 M PBS and 50 ng/mL in corn flour samples, meanwhile α-ZOL, β-ZOL, and ZEA were found to be 1 ng/mL in 0.01 M PBS and 75 ng/mL in corn flour samples. Our data indicate that the ICA is sensitive, rapid, cost-effective, and specific on-site screening for ZEN and its metabolite.

【 授权许可】

CC BY