Immunochromatographic strip for ultrasensitive detection of fumonisin B1

Food and Agricultural Immunology

Qiankun Zheng¹  Tao Hong²  Kai Hao³  Hua Kuang⁴  Shanshan Song⁴  Steven Suryoprabowo⁴  Liqiang Liu⁴ 

[1] Delishi Group, Weifang, Shandong, People’s Republic of Chin;National Institute of Metrology, Beijing, People’s Republic of Chin;School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, People’s Republic of Chin;State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of Chin;International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People’s Republic of Chin;

关键词: Fumonisins;    Fumonisin B;    immunochromatographic assay;    monoclonal antibody;    strip test;   

DOI  :  10.1080/09540105.2018.1439455

来源: publisher

【 摘 要 】

A sensitive monoclonal antibody (mAb) (1H1) against fumonisin B1 (FB1) was produced in our laboratory. A rapid, simple, and an immunochromatographic strip has been developed for the ultrasensitive detection of FB1 in corn samples. Under the optimized condition, the cut-off limits of test strips for FB1 were found to be 50 ng/mL in 0.01 M PBS and 25 ng/mL in corn samples. Results could be obtained within 5 min. The results revealed that the developed method is a rapid, sensitive, and simple tool for the detection of FB1.

【 授权许可】

CC BY