Pectinase and polygalacturonase production by a thermophilic Aspergillus fumigatus isolated from decomposting orange peels

Brazilian Journal of Microbiology

Urmila Phutela¹  Vikram Dhuna¹  Shobhna Sandhu¹  B.s. Chadha¹ 

[1] ,Guru Nanak Dev UniversityAmritsar Punjab ,India

关键词: thermophilic fungi;    Aspergillus fumigatus;    pectinase;    polygalacturonase;    fungos termofílicos;    Aspergillus fumigatus;    pectinase;    poligalacturonase;   

DOI  :  10.1590/S1517-83822005000100013

来源: SciELO

【 摘 要 】

A thermophilic fungal strain producing both pectinase and polygalacturonase was isolated after primary screening of 120 different isolates. The fungus was identified as Aspergillus fumigatus Fres. MTCC 4163. Using solid-state cultivation, the optimum levels of variables for pectinase and polygalacturonase (PG) production were determined. Maximal levels of enzyme activities were achieved upon growing the culture in a medium containing wheat bran, sucrose, yeast extract and (NH4)2SO4 after 2-3 days of incubation at a temperature of 50ºC. Highest enzyme activities of 1116 Ug-1 for pectinase and 1270 Ug-1 for polygalacturonase were obtained at pH 4.0 and 5.0, respectively.

【 授权许可】

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