Brazilian Journal of Microbiology | |
Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans | |
Renata I. Barense1  Maria Angélica Dos S.c. Chellegatti1  Maria José V. Fonseca1  Suraia Said1  | |
[1] ,Universidade de São Paulo Faculdade de Ciências Farmacêuticas de Ribeirão Preto Departamento de Ciências FarmacêuticasRibeirão Preto SP ,Brasil | |
关键词: Exopolygalacturonase; pectinase; polygalacturonase; Penicillium frequentans; Pectinase; exopoligalacturonase; poligalacturonase; Penicillium frequentans; | |
DOI : 10.1590/S1517-83822001000400014 | |
来源: SciELO | |
【 摘 要 】
Previous studies from our laboratory have demonstrated that the fungus Penicillium frequentans produces high levels of polygalacturonase and pectinesterase. Endopolygalacturonase I (Endo-PG I) and Exopolygalacturonase I (Exo-PG I) were previously purified and characterized. In the present study two extracellular polygalacturonases were separated, partially purified and biochemically characterized. Both were characterized as exopolygalacturonases so they were named exopolygalacturonase II (Exo-PG II) and exopolygalacturonase III (Exo-PG III) which had a molecular mass of 63 kDa (Exo-PG II) and 79 kDa (Exo-PG III). The Km values were 1.6 and 0.059 g/L and the Vmax values were 2571 and 185 U/mg, respectively. The optimum temperature was 50ºC for both enzymes, while the optimum pH was 5.0 for Exo-PG II and 5.8 for Exo-PG III.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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