Molecular characterization of enteroinvasive Escherichia coli ipa genes by PCR-RFLP analysis

Brazilian Journal of Microbiology

Adriana Gibotti²  Tânia L. Tanaka¹  Valéria R. Oliveira²  Carla R. Taddei²  Marina B. Martinez² 

[1] ,Universidade de São Paulo Instituto de Ciências Biomédicas Departamento de MicrobiologiaSão Paulo SP ,Brasil

关键词: ipa;    enteroinvasive Escherichia coli;    polymorphism;    PCR-RFLP;    ipa;    Escherichia coli enteroinvasora;    polimorfismo;    PCR-RFLP;   

DOI  :  10.1590/S1517-83822004000100012

来源: SciELO

【 摘 要 】

In this study, polymorphism in ipa genes was found in five out of nine EIEC serotypes studied. When SalI and HindII were used in RFLP-PCR assays many EIEC serotypes showed polymorphism in ipaB and ipaD. On the other hand, no polymorphism was observed in ipaA and ipaC in these strains. The polymorphism present in EIEC strains is serotype-dependent, since restriction patterns were conserved amongst strains belonging to the same serotype. When IpaB deduced amino acid sequences of S. flexneri M90T and FBC124-13 were compared, ten amino acids changes could be observed mainly in the amino-terminal region. The deduced EIEC IpaD amino-acid sequence presented 91% similarity with the Shigella strain. In this case, amino acid changes were spread out through the whole structure, except in the carboxyl-terminal region.

【 授权许可】

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