Polysaccharide production in batch process of Neisseria meningitidis serogroup C comparing Frantz, modified Frantz and Cartlin 6 cultivation media

Brazilian Journal of Microbiology

Marcelo Fossa Da Paz¹  Júlia Baruque-ramos¹  Haroldo Hiss¹  Márcio Alberto Vicentin¹  Maria Betania Batista Leal¹  Isaías Raw¹ 

[1] ,Instituto Butantan Centro de Biotecnologia São Paulo SP ,Brasil

关键词: Neisseria meningitidis;    culture medium;    vaccine;    polysaccharide;    batch cultivation;    Neisseria meningitidis;    meio de cultura;    vacina;    polissacarídeo;    cultivo descontínuo;   

DOI  :  10.1590/S1517-83822003000100007

来源: SciELO

【 摘 要 】

Polysaccharide of N. meningitidis serogroup C constitutes the antigen for the vaccine against meningitis. The goal of this work was to compare three cultivation media for production of this polysaccharide: Frantz, modified Frantz medium (with replacement of glucose by glycerol), and Catlin 6 (a synthetic medium with glucose). The comparative criteria were based on the final polysaccharide concentrations and the yield coefficient cell/polysaccharide (Y P/X). The kinetic parameters: pH, substrate consumption and cell growth were also determined. For this purpose, 9 cultivation runs were carried out in a 80 L New Brunswick bioreactor, under the following conditions: 42 L of culture medium, temperature 35ºC, air flow 5 L/min, agitation frequency 120 rpm and vessel pressure 6 psi, without dissolved oxygen or pH controls. The cultivation runs were divided in three groups, with 3 repetitions each. The cultivation using the Frantz medium presented the best results: average of final polysaccharide concentration = 0.134 g/L and Y P/X=0.121, followed by Catlin 6 medium, with results of 0.095 g/L and 0.067 respectively. Considering the principal advantages in the use of the synthetic medium, i.e. facilitation of a cultivation and purification steps of the polysaccharide production process, there is a possibility that in the near future, Catlin 6 will replace the traditional Frantz medium.

【 授权许可】

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