Influence of initial L-asparagine and glycerol concentrations on the batch growth kinetics of Mycobacterium bovis BCG

Brazilian Journal of Microbiology

Maria Betania Batista Leal¹  Júlia Baruque-ramos¹  Haroldo Hiss¹  Marcelo Fossa Da Paz¹  Maria Cristina Sakai¹  Umbelina Macedo Vassoler¹  Luciana Juncioni De Arauz¹  Isaías Raw¹ 

[1] ,Instituto Butantan Centro de Biotecnologia São Paulo SP ,Brasil

关键词: BCG;    Mycobacterium bovis;    culture medium;    vaccine;    submerged batch cultivation;    BCG;    Mycobacterium bovis;    meio de cultura;    vacina;    cultivo submerso descontínuo;   

DOI  :  10.1590/S1517-83822004000300013

来源: SciELO

【 摘 要 】

The influences of the L-asparagine and glycerol initial concentrations in Sauton medium on the productivities of biomass and colony forming units were studied. The submerged batch cultivations of Mycobacterium bovis were carried out in a 20 L bioreactor. The L-asparagine and glycerol initial concentrations of 4.54 g/L and 25 mL/L, respectively, corresponded to the best biomass productivity, namely 2.5 g/L.day. On the other hand, the concentrations of 2.27 g/L and 25 mL/L, respectively, led to the highest productivity in terms of colony forming units, namely 2.7·10(6) colonies/mg.day. In addition, by means of the relative consumption analysis of L-asparagine and glycerol (50 and 26% respectively), it was concluded that the concentrations of such components could be reduced, with respect to the original Sauton medium composition, aiming the obtainment of an optimal BCG vaccine production in the bioreactor.

【 授权许可】

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