Brazilian Journal of Microbiology | |
The synthesis of Phosphate-repressible alkaline phosphatase do not appear to be regulated by ambient pH in the filamentous mould Neurospora crassa | |
Sérgio R. Nozawa2  Geraldo Thedei Jr.1  Luciana S.p. Crott1  José E. Barbosa1  Antonio Rossi1  | |
[1] ,Universidade de São Paulo Faculdade de Filosofia, Ciências e Letras de Ribeirão PretoFaculdade de Filosofia, Ciências e Letras de Ribeirão PretoFaculdade de Filosofia, Ciências e Letras de Ribeirão Preto Departamento de QuímicaRibeirão Preto SP ,Brasil | |
关键词: alkaline phosphatase; ambient pH; enzyme secretion; Neurospora crassa; Pi sensing; fosfatase alcalina; Neurospora crassa; pH ambiente; secreção de enzimas; | |
DOI : 10.1590/S1517-83822002000100018 | |
来源: SciELO | |
【 摘 要 】
In order to investigate further the adaptive response of moulds to ambient pH, we have measured by ELISA the pho-2-encoded Pi-repressible alkaline phosphatase synthesised by Neurospora crassa. We showed that the 74A and pho-2A strains of this mould secrete similar amounts of the pho-2-encoded enzyme irrespective of ambient pH, when both the preg and pgov genes are not functional, i.e., in strains nuc-2+ growing under Pi-starvation. This suggests that pho-2, which is responsive to Pi starvation via the action of genes nuc-2, preg, pgov and nuc-1, is not a gene responsive to ambient pH and that the differential glycosylation observed for the Pi-repressible alkaline phosphatase retained by the mycelium at pH 5.6 or secreted into the growth medium at pH 8.0 is the genetic response to ambient pH sensing in N. crassa.
【 授权许可】
CC BY-NC
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202005130167088ZK.pdf | 52KB | download |