The synthesis of Phosphate-repressible alkaline phosphatase do not appear to be regulated by ambient pH in the filamentous mould Neurospora crassa

Brazilian Journal of Microbiology

Sérgio R. Nozawa²  Geraldo Thedei Jr.¹  Luciana S.p. Crott¹  José E. Barbosa¹  Antonio Rossi¹ 

[1] ,Universidade de São Paulo Faculdade de Filosofia, Ciências e Letras de Ribeirão PretoFaculdade de Filosofia, Ciências e Letras de Ribeirão PretoFaculdade de Filosofia, Ciências e Letras de Ribeirão Preto Departamento de QuímicaRibeirão Preto SP ,Brasil

关键词: alkaline phosphatase;    ambient pH;    enzyme secretion;    Neurospora crassa;    Pi sensing;    fosfatase alcalina;    Neurospora crassa;    pH ambiente;    secreção de enzimas;   

DOI  :  10.1590/S1517-83822002000100018

来源: SciELO

【 摘 要 】

In order to investigate further the adaptive response of moulds to ambient pH, we have measured by ELISA the pho-2-encoded Pi-repressible alkaline phosphatase synthesised by Neurospora crassa. We showed that the 74A and pho-2A strains of this mould secrete similar amounts of the pho-2-encoded enzyme irrespective of ambient pH, when both the preg and pgov genes are not functional, i.e., in strains nuc-2+ growing under Pi-starvation. This suggests that pho-2, which is responsive to Pi starvation via the action of genes nuc-2, preg, pgov and nuc-1, is not a gene responsive to ambient pH and that the differential glycosylation observed for the Pi-repressible alkaline phosphatase retained by the mycelium at pH 5.6 or secreted into the growth medium at pH 8.0 is the genetic response to ambient pH sensing in N. crassa.

【 授权许可】

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