Use of Readycult™ - LMX for enumeration of total coliforms and Escherichia coli in milk

Brazilian Journal of Microbiology

Vanerli Beloti¹  Márcia De Aguiar Ferreira Barros¹  Maurício Pinto Nunes¹  Elsa Helena Walter De Santana¹  Luís Augusto Nero¹  Juliana Aparecida De Souza¹ 

[1] ,Universidade Estadual de Londrina Departamento de Medicina Veterinária Preventiva Laboratório de Inspeção de Produtos de Origem Animal

关键词: Readycult™ - LMX;    X-GAL;    MUG;    milk;    Escherichia coli;    total coliforms;    Readycult™ - LMX;    X-GAL;    MUG;    leite;    Escherichia coli;    coliformes totais;   

DOI  :  10.1590/S1517-83822002000100010

来源: SciELO

【 摘 要 】

Readycult™ - LMX is a rapid method to test for the presence of total coliform bacteria and Escherichia coli in water, giving results in 24h. It is based on reactions of specific microbial enzymes or indicator nutrients of the medium. The goal of this paper was to study the use of Readycult™ - LMX to detect total coliform and E. coli in milk. One hundred twenty five samples of pasteurized and raw milk, collected in Londrina, PR, Brazil, were tested simultaneously by the most probable number (MPN) method using Brilliant Green Bile Lactose Broth (2%) (BRILA) and Readycult™ - LMX. The Readycult™ - LMX test was evaluated for sensitivity, simplicity, and speed of results. There was a significant correlation between the results obtained by the two methods for total coliforms (r: 0.8224) and for E. coli (r: 0.8603). The two methods yielded similar results, but Readycult™ - LMX was easier to use. In addition, results were available as early as 24h.

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