C‐terminal end of v‐src protein interacts with peptide coded by gadd7/adapt15‐like RNA in two‐hybrid system

FEBS Letters

Tatosyan, Alexander¹  Camonis, Jacques²  Tavitian, Armand²  Mizenina, Olga¹  Musatkina, Elena¹  Yanushevich, Yuriy¹  Rodina, Anna¹ 

[1] Institute of Carcinogenesis, Cancer Research Center, Kashirskoye shosse, 24, 115478 Moscow, Russia;INSERM, U.248, Institut Curie, 28 rue d'Ulm, 75248, Paris Cedex 05, France

关键词: v-src;    Metastasis;    vseap1;    gadd7;    Two-hybrid system;    Hamster cell;    ORF;    open reading frame;    aa;    amino acid(s);    GST;    glutatione S-transferase;    PCR;    polymerase chain reaction;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    ECL;    chemiluminescence detection system;    PMSF;    phenylmethylsulfonyl fluoride;    IPTG;    isopropyl-β-d-thiogalactopyranoside;    X-GAL;    5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside;   

DOI  :  10.1016/S0014-5793(97)01568-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The significant differences in the metastatic properties of hamster fibroblasts transformed by the Rous sarcoma virus (RSV) were associated with mutations in the v-src carboxy-terminal region. To identify the capacity of this region for protein–protein interaction the two-hybrid system was used. The cDNA clone (vseap1), producing the protein specifically bound with the v-src C-terminal part in yeast cells in vivo and in GST-fusion system in vitro was isolated. Vseap1 shared 68% of homology with stressful agents induced RNA-gadd7/adapt15. Two vseap1 specific messenger RNAs were identified: 0.9-kbp RNA expressed in all transformed cells and three times less in embryo fibroblasts; 3.1-kbp transcript was deleted in the cells with suppressed v-src activity and H₂O₂ resistance.

【 授权许可】

Unknown