Different culture media applied to the study of Cryptococcus neoformans susceptibility to amphotericin B and fluconazole

Brazilian Journal of Microbiology

Sydney Hartz Alves²  Loiva T. Oliveira¹  Letícia S. Goulart¹  Carlos Eduardo B. Linares¹  Josiane Griebeler¹  Janio M. Santurio¹ 

[1] ,Universidade Federal de Santa Maria Departamento Análises Clínicas e Toxicológicas Laboratório de Pesquisas MicológicasSanta Maria RS ,Brasil

关键词: Cryptococcus neoformans;    susceptibility;    antifungal agents;    Cryptococcus neoformans;    suscetibilidade;    antifúngicos;   

DOI  :  10.1590/S1517-83822002000100005

来源: SciELO

【 摘 要 】

This study compared the performance of three culture media used for the determination of minimal inhibitory concentration (MIC) of amphotericin B and fluconazole against 48 Cryptococcus neoformans strains. For amphotericin B the media employed were RPMI 1640 medium, as indicated by NCCLS as reference, Yeast Nitrogen Base (YNB) as proposed by Ghannoum et al. (YNB-1), Antibiotic medium 3 (AM3) indicated by others, and YNB as proposed by us (YNB-2). In YNB-1, amphotericin B showed broader MIC ranges, varying from 0.125 µg/ml to 1.0 µg/ml; in RPMI 1640 medium and YNB-2 the MICs were similar and in AM3 the MIC ranges were narrower. The same pattern was observed for MIC 90%. For fluconazole the media employed were RPMI 1640 and YNB-1. The MIC ranges were broader and elevated when YNB-1 was employed. MIC 50% were 2.0 µg/ml in RPMI 1640 medium and 4.0 µg/ml in YNB-1. Our results indicate that RPMI 1640 supplemented with 2% glucose allowed a good growth at 48h; YNB as recommended by Ghannoum et al. provided a broader MIC range, AM3 provided a narrow MIC range for amphotericin B, and YNB-2 was very similar to RPMI 1640 medium.

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