Culture conditions for the production of thermostable amylase by Bacillus sp

Brazilian Journal of Microbiology

Carlos Eduardo De Souza Teodoro¹  Meire Lelis Leal Martins¹ 

[1],Universidade Estadual do Norte Fluminense Centro de Ciências e Tecnologias Agropecuárias Campos RJ ,Brasil

关键词: alpha-amylase;    thermophilic bacterium;    thermostable enzymes;    Bacillus sp;    alfa-amilase;    bactéria termofílica;    enzimas termoestáveis;    Bacillus sp;   

DOI  :  10.1590/S1517-83822000000400011

来源: SciELO

【 摘 要 】

Studies on the alpha-amylase production were carried out with a bacterial strain isolated from a soil sample. The cells were cultivated in a mineral medium containing soluble starch as sole carbon source. The addition of calcium (10 mM) or peptone (1%) and yeast extract (0.5%) to the mineral medium shortened the lag period and improved the growth and alpha-amylase synthesis. The addition of glucose to the culture diminished greatly the synthesis of alpha-amylase, demonstrating that a classical glucose effect is operative in this organism. The optimum temperature and initial medium pH for amylase synthesis by the organism were 50°C and 7.0 respectively. The optimal pH and temperature for activity were 6.0 and 50°C respectively. The enzyme extract retained 100% activity when incubated for one hour at 90°C and 40% at 60°C for 24 h. The addition of glucose to the culture diminished greatly the synthesis of alpha-amylase.

【 授权许可】

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