Effect of the medium composition on formation of amylase by Bacillus sp

Brazilian Archives of Biology and Technology

Eliana De Oliveira. Santos¹  Meire Lelis Leal Martins¹ 

[1] ,Universidade Estadual do Norte Fluminense Centro de Ciências e Tecnologias Agropecuárias Campos RJ ,Brazil

关键词: alpha-amylase;    thermophilic bacterium;    Bacillus sp;   

DOI  :  10.1590/S1516-89132003000100018

来源: SciELO

【 摘 要 】

Studies on the alpha -amylase synthesis was carried out with a moderately thermophilic, facultatively anaerobic Bacillus sp, isolated from soil samples. The cells were cultivated in a complex medium containing soluble starch or maltose as carbon source. The levels of the alpha -amylaseactivity detected in culture supernatants varied greatly with the type of carbon source used. Maltose, soluble starch and citrate stimulated alpha -amylaseformation. Addition of exogenous glucose repressed formation of alpha -amylase, demonstrating that a classical glucose effect was operative in this organism. The concentration of yeast extract was found to be important factor in the alpha -amylase synthesis bythe isolate.The activity of the enzyme increased between 2 and 5 g/L yeast extract concentration and then fell very rapidly beyond this point. The best concentration of peptone to alpha-amylase formation was found to be around 10g/L.

【 授权许可】

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