Elaboration and evaluation of a new screening medium for detection and presumptive identification of extended-spectrum beta-lactamase-producing organisms (ESBL)

Brazilian Journal of Microbiology

Carlos Henrique Pessôa De Menezes E Silva¹ 

[1] ,Marcos Daniel Laboratório Setor de Microbiologia Vitória ES ,Brasil

关键词: Enterobacteriaceae;    culture medium;    ESBL;    enterobactérias;    meio de cultura;    ESBL;   

DOI  :  10.1590/S1517-83822000000400006

来源: SciELO

【 摘 要 】

The new ß-lactamases have arisen largely as a consequence of heavy use of new expanded spectrum ß-lactam antibiotics. Health professionals need to be aware of the resistance problems caused by the new enzymes, and know the correct procedures to detect, prevent and control such problems. In this study, a new screening medium called Ceftazidime-Inositol-Vancomycin-Amphotericin B Agar (CIVA) was elaborated and the microbiological performance was evaluated for the detection and presumptive identification of ESBL-producing members of Enterobacteriaceae. It was performed in 126 stool samples from hospitalized patients at Santa Monica Hospital (Vila Velha, ES, Brazil), who had been heavily exposed to broad-spectrum antibiotic combinations. The bacteria were detected by the medium based on their colony colours (due to inositol fermentation). Additional tests were required for correct identification of these strains. No false positive rates were detected.

【 授权许可】

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