Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures

Brazilian Archives of Biology and Technology

Angelo Luis Caron¹  Virgínia Picanço-castro¹  Sven Ansorge¹  Dimas Tadeu Covas¹  Amine Kamen¹  Kamilla Swiech¹ 

关键词: Factor VIII;    Hek293 cells;    lentiviral vectors;    polyethylenimine;    serum-free suspension culture;    transient transfection;   

DOI  :  10.1590/S1516-89132015060367

来源: SciELO

【 摘 要 】

ABSTRACT Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles.

【 授权许可】

CC BY-NC   
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