Combined Application of RGB Marking and Mass Spectrometric Imaging Facilitates Detection of Tumor Heterogeneity

Cancer Genomics - Proteomics

PIERRE ABRAMOWSKI³  SASCHA ROHN²  KRISTOFFER RIECKEN³  BORIS FEHSE³  HARTMUT SCHLÜTER¹  OLGA KRAUS¹ 

[1]ass Spectrometric Proteome Analysis, Department of Clinical Chemistry and Laboratory Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germanyass Spectrometric Proteome Analysis, Department of Clinical Chemistry and Laboratory Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germanyass Spectrometric Proteome Analysis, Department of Clinical Chemistry and Laboratory Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

[2]amburg School of Food Science, Institute of Food Chemistry, University of Hamburg, Hamburg, Germanyamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, Hamburg, Germanyamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, Hamburg, Germany

[3]esearch Department of Cell and Gene Therapy, Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germanyesearch Department of Cell and Gene Therapy, Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germanyesearch Department of Cell and Gene Therapy, Department of Stem Cell Transplantation, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

关键词: Mass spectrometric imaging;    MSI;    tumor heterogeneity;    RGB marking;    proteomics;    lentiviral vectors;   

DOI  :  

来源: Delinasios GJ CO

【 摘 要 】

Cancer-cell heterogeneity dramatically influences treatment success, but escapes detection by classical histology. Mass-spectrometric imaging (MSI) represents a powerful method for visualizing the spatial distribution of proteins in tissue sections. Herein we asked whether MSI also facilitates detection of tumor heterogeneity. We first transduced the human neuroendocrine-carcinoma BON cell line following the red-green-blue (RGB) marking principle. RGB marking allows for specific color-coding of individual clones. Mice transplanted with RGB-marked BON cells developed liver tumors. We identified 16 primary tumors clearly distinguishable by histology and fluorescence imaging, but also based on a common tumor-specific signal pattern detected by MSI. Importantly, this pattern was clearly confined to tumor tissue while was absent from surrounding liver tissue. At the same time, we observed protein signals differentially present in a few or even single tumors. Since these signals were independent of RGB marking, they apparently reflected unique intrinsic protein-signal patterns of individual tumors. Thus, our data propose MSI as a tool for identifying divergent tissue by ‘fingerprints’ of protein signals, allowing not only for differentiation of tumor from healthy tissue but also detection of tumor heterogeneity. In conclusion, by visualizing tumor heterogeneity, MSI ideally complements microscopy-based methods. This might help to better understand tumor biology and develop future treatment strategies.

【 授权许可】

Unknown