Adsorption, immobilization and activity of cellulase in soil: the impacts of maize straw and its humification

Brazilian Archives of Biology and Technology

Ali Akbar Safari Sinegani¹  Mahboobe Safari Sinegani¹ 

[1] ,Bu-Ali Sina University Faculty of Agriculture Hamedan,Iran

关键词: Maize straw;    Humification;    Cellulase;    Sorption;    Immobilization;    Activity;   

DOI  :  10.1590/S1516-89132013005000006

来源: SciELO

【 摘 要 】

The present work aimed to study some aspects of sorption and immobilization of cellulase molecules on soil components by the analysis of the reactions of cellulase in a soil treated with different levels of maize residue and incubated for 90 days. The analysis of variance showed that the effects of the treatments of maize straw, incubation time and their interaction on cellulase adsorption, desorption and immobilization were statistically significant. The adsorption and immobilization capacities of soil by application of maize straw increased significantly. However they decreased with decreasing the soil organic matter (SOM) after 45 days of incubation. The desorption of adsorbed cellulase molecules from the soil by washing with distilled water depended on the SOM contents and its humification. The binding strength of cellulase molecule with fresh miaze straw was significantly stronger than that with humified maize straw. The immobilized cellulase activity, particularly its specific activity increased significantly by increasing the OC contents in the soil treated with maize straw.

【 授权许可】

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