In Vivo investigation of radiolabeled Bevacizumab in healthy rat tissues

Brazilian Archives of Biology and Technology

Ilknur Demir¹  Fazilet Zumrut Biber Muftuler¹  Perihan Unak¹  Cigdem Acar¹ 

[1] ,Ege University Institute of Nuclear Sciences Department of Nuclear ApplicationsBornova Izmir ,Turkey

关键词: Angiogenesis;    Bevacizumab;    99mTc;   

DOI  :  10.1590/S1516-89132011000100010

来源: SciELO

【 摘 要 】

In this study, BevMab was conjugated with the bifunctional chelating agent [diethylenetriamine pentaacetic acid (DTPA)] and the product (BevMab-DTPA) was labeled with 99mTc using stannous chloride reducing method. The quality control studies of radiolabeled compound (99mTc-BevMab-DTPA) were done with Thin Layer Radio Chromatography (TLRC) and High Performance Liquid Radio Chromatography (HPLRC) methods (% 95 <) to confirm the labeling efficiency. High radiochemical yield [98.07 % ± 2.17 (n = 13)] was obtained by TLRC method. Biodistribution studies of 99mTc labeled BevMab-DTPA was run on healthy female and male Albino Wistar rats. The distribution figures demonstrated that the radiolabeled compound was eliminated through the kidneys and accumulated in urinary bladder. The values of the BevMab-DTPA uptakes were similar in heart, blood, liver and spleen in both sexes.

【 授权许可】

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