Development of an algorithm for tip-related artifacts identification in AFM biological film imaging

Brazilian Archives of Biology and Technology

Rubens Bernardes-filho¹  Odilio Benedito Garrido De Assis¹ 

[1] ,Embrapa Instrumentação AgropecuáriaSão Carlos SP ,Brasil

关键词: Probe microscopy;    AFM;    images tip artifacts;    biological film;   

DOI  :  10.1590/S1516-89132005000500020

来源: SciELO

【 摘 要 】

One major drawback identified in atomic force microscopy imaging is the dependence of the image's precision on the shape of the probe tip. In this paper a simple algorithm is proposed to provide artifact identification signaling in-situ tip features in atomic force microscopy images. The base of the identifications lied when the angle formed between two scanned points was kept the same as the tip sweeps a certain length of the sample. The potential of the described method was illustrated on a chitosan polysaccharide film. The images produced were compared to evaluate tip-artifact regions. This algorithm showed promise as a tool in the measurement and characterization fields to separate true images from artificial images in probe microscopy.

【 授权许可】

CC BY-NC   
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