Cellulase activity of a Lentinula edodes (Berk.) Pegl. strain grown in media containing Carboximetilcellulose or microcrystalline cellulose

Brazilian Archives of Biology and Technology

José Antônio De Sousa Pereira Júnior²  Marcos José Correia¹  Neiva Tinti De Oliveira² 

[1] ,Universidade Federal de Pernambuco Centro de Ciências Biológicas Departamento de MicologiaRecife PE ,Brazil

关键词: Lentinula edodes;    cellulase;    liquid medium cultivation;   

DOI  :  10.1590/S1516-89132003000300004

来源: SciELO

【 摘 要 】

Endoglucanase and exocellobiohydrolase produced b Lentinula edodes (Berk.) Pegl. strain thatt was cultivated in carboxymetilcellulose (CMC) or microcrystalline cellulose (Avicel) liquid media. The concentration and type of cellulose influenced the enzyme activity and production. Extra-cellular cellobiase activity was not detected in CMC or Avicel media. This enzyme was detected in mycelial extracts only. With 1.7% Avicel liquid medium, the strain did not produce exocellobiohydrolase, but 74 µmol RBBR/mg protein/min was detected with 0.5% Avicel. The substitution of Avicel by 0.5% CMC reduced this activity. Endoglucanase also had maximum activity in 0.5% Avicel medium (approximately 820 UI/mg protein) after 96 h incubation. In supernatants from 0.5% CMC, the maximum activity attained was 200 UI/mg protein only.

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