Polysaccharide and extracts from Lentinula edodes: structural features and antiviral activity

Virology Journal

Rosa Elisa Carvalho Linhares²  Carlos Nozawa²  Luzia Doretto Paccola Meirelles³  Sandra Aguiar Soares¹  Nágila Maria Pontes Silva Ricardo¹  Kristie Aimi Yamamoto²  Vinicius Pires Rincão² 

[1] Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Av. da Universidade, 2853, CEP: 60455-760, Fortaleza, Ceará, Brazil;Departamento de Microbiologia, Universidade Estadual de Londrina, Rod. Celso Garcia Cid., km 380, CEP: 86051-990, Londrina, Paraná, Brazil;Departamento de Biologia Geral, Universidade Estadual de Londrina, Rod. Celso Garcia Cid., km 380, CEP: 86051-990, Londrina, Paraná, Brazil

关键词: Bovine herpesvirus;    Poliovirus;    Antiviral activity;    Lentinula edodes;   

Others  :  1155103
DOI  :  10.1186/1743-422X-9-37

 received in 2010-11-30, accepted in 2012-02-15,  发布年份 2012

【 摘 要 】

Background

Lentinula edodes, known as shiitake, has been utilized as food, as well as, in popular medicine, moreover, compounds isolated from its mycelium and fruiting body have shown several therapeutic properties. The aim of this study was to determine the antiviral activity of aqueous (AqE) and ethanol (EtOHE) extracts and polysaccharide (LeP) from Lentinula edodes in the replication of poliovirus type 1 (PV-1) and bovine herpes virus type 1 (BoHV-1).

Methods

The time-of-addition assay was performed at the times -2, -1, 0, 1 and 2 h of the infection. The virucidal activity and the inhibition of viral adsorption were also evaluated. Plaque assay was used to monitor antiviral activity throughout.

Results

The AqE and LeP were more effective when added at 0 h of infection, however, EtOHE was more effective at the times 1 h and 2 h of the infection. AqE, EtOHE and LeP showed low virucidal activity, and the inhibition of viral adsorption was not significant.

Conclusions

The results allowed us to conclude that AqE, EtOHE and LeP act on the initial processes of the replication of both strains of virus.

【 授权许可】

   
2012 Rincão et al; BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

Figure 4.

【 参考文献 】

• [1]De Clercq E, Field HJ: Antiviral prodrugs--the development of successful prodrug strategies for antiviral chemotherapy. Br J Pharmacol 2006, 147:1-11.
• [2]Jones PS: Strategies for antiviral drug discovery. Antivir Chem Chemother 1998, 9:283-302.
• [3]Strohl WR: The role of natural products in a modern drug discovery program. Drug Discov Today 2000, 5:39-41.
• [4]Jiang T, Luo S, Chen Q, Shen L, Ying T: Effect of integrated application of gamma irradiation and modified atmosphere packaging on physicochemical and microbiological properties of shiitake mushroom (Lentinus edode). Food Chem 2010, 122:761-767.
• [5]Sugui MM, De Lima PLA, Delmanto RD, Da Eira AF, Salvadori DMF, Ribeiro LR: Antimutagenic effect of Lentinula edode (BERK.) Pegler mushroom and possible variation among lineages. Food Chem Toxicol 2003, 41:555-560.
• [6]Kupfahl C, Geginat G, Hof H: Lentinan has a stimulatory effect on innate and adaptive immunity against murine Listeria monocytogene infection. Int Immunopharmacol 2006, 6:686-696.
• [7]Suzuki M, Takatsuki F, Maeda YY, Hamuro J, Chihara G: Antitumor and immunological activity of Lentinan in comparision with LPS. Int J Immunopharmacol 1994, 16:463-468.
• [8]Maruyama S, Sukekawa Y, Kaneko Y, Fujimoto S: Anti-tumor activities of lentinan and micellapist in tumor-bearing mice. Gan To Kagaku Ryoho 2006, 33:1726-1729.
• [9]Zhang L, Li X, Xu X, Zeng F: Correlation between antitumor activity, molecular weight, and conformation of lentinan. Carbohydr Res 2005, 340:1515-1521.
• [10]Jong SC, Birmingham M: Medicinal and therapeutic value of the shiitake mushroom. Adv Appl Microbiol 1993, 39:153-184.
• [11]Markova N, Kussovski V, Drandarska I, Nikolaeva S, Georgieva N, Radoucheva T: Protective activity of Lentinan in experimental tuberculosis. Int Immunopharmacol 2003, 3:1557-1562.
• [12]Kaneko Y, Chihara G: Potentiation of host resistance against microbial infections by Lentinan and its related polysaccharides. In Microbial Infections. Edited by Friedman H et al. New York: Plenum; 1992:201-206.
• [13]Sasaki SH, Linhares REC, Nozawa CM, Montalván R, Paccola-Meirelles LD: Strains of Lentinula edode suppress growth of phytopathogenic fungi and inhibit Alagoas serotype of vesicular stomatitis virus. Braz J Microbiol 2001, 32:52-55.
• [14]Wang S, Welte T, Fang H, et al.: Oral Administration of Active Hexose Correlated Compound Enhances Host Resistance to West Nile Encephalitis in Mice. J Nutr 2009, 139:598-602.
• [15]Racaniello VR: One hundred years of poliovirus pathogenesis. Virology 2006, 344:9-16.
• [16]World Health Organization (WHO): Global polio eradication initiative. http://www.polioeradication.org/Dataandmonitoring/Poliothisweek.aspx webcite. Accessed 26 dec 2011.
• [17]Jones C, Geiser V, Henderson G, et al.: Functional analysis of bovine herpesvirus 1 (BHV-1) genes expressed during latency. Vet Microbiol 2006, 113:199-210.
• [18]Hurk SDL, Loehr BI, Babiuk LA: Immunization of livestock with DNA vaccines: current studies and future prospects. Vaccine 2001, 19:2474-2479.
• [19]Gonzaga MLC, Ricardo NMPS, Heatley F, Soares SA: Isolation and characterization of polysaccharides from Agaricus blaze Murill. Carbohydr Polym 2005, 60:43-49.
• [20]Melo FL, Benati FJ, Roman WA Jr, Mello JCP, Nozawa C, Linhares REC: The in vitro antiviral activity of an aliphatic nitrocompound from Heteropteris aphrodisiac. Microbiol Res 2008, 163:136-139.
• [21]Yang CM, Cheng HY, Lin TC, Chiang LC, Lin CC: Acetone, ethanol and methanol extracts of Phyllanthus urinari inhibit HSV-2 infection in vitro. Antiviral Res 2005, 67:24-30.
• [22]Zhu W, Chiu LCM, Ooi VEC, Chan PKS, Ang PO Jr: Antiviral property and mode of action of a sulphated polysaccharide from Sargassum paten against herpes simplex virus type 2. Int J Antimicrob Agents 2004, 24:81-85.
• [23]Sorimachi K, Ikehara Y, Maezato G, et al.: Inhibition by Agaricus blaze Murrill fractions of cytopathic effect induced by Western Equine Encephalitis (WEE) Virus on VERO cells in vitro. Biosci Biotechnol Biochem 2001, 65:1645-1647.
• [24]Awadh Ali NA, Mothana RAA, Lesnau A, Pilgrim H, Lindesquist U: Antiviral activity of Inonotus hispidu. Fitoterapia 2003, 74:483-485.
• [25]Surenjav U, Zhang L, Xu X, Zhang X, Zeng F: Effects of molecular structure on antitumor activities of (1 → 3)--d-glucans from different Lentinus Edode. Carbohydr Polym 2006, 63:97-104.
• [26]Zhang Y, Li S, Wang X, Zhang L, Cheung PCK: Advances in lentinan: Isolation, structure, chain conformation and bioactivities. Food Hydrocolloid 2011, 25:196-206.
• [27]Eo SK, Kim YS, Oh KW, Lee CK, Lee YN, Han SS: Mode of Antiviral Activity of Water Soluble Components Isolated from Elfvingia applanat on Vesicular Stomatitis Virus. Arch Pharm Res 2001, 24:74-78.
• [28]Kabanov A, Sementsova AO, Skarnovich MO, Teplyakova TV, Shishkina LN, Sergeev NA: Development of new effective antiinfluenza drugs based on extracts of basidiomycetes. Int J Infect Dis 2010, 14:(Suppl 1):e88.
• [29]Damonte EB, Matulewicz MC, Cerezo AS: Sulfated seaweed polysaccharides as antiviral agents. Curr Med Chem 2004, 11:2399-2419.