Effects of the spermatozoa: oocyte ratio, water volume and water temperature on artificial fertilization and sperm activation of cascudo-preto

Revista Brasileira de Zootecnia

Robie Allan Bombardelli²  Eduardo Antônio Sanches¹  Diego Mendes Baggio²  Renata Marino Sykora²  Bruno Estevão De Souza¹  Lucélia Tessaro¹  Pitágoras Augusto Piana² 

[1] ,Universidade Estadual do Oeste do Paraná Curso de Engenharia de Pesca Toledo Paraná ,Brazil

关键词: fish;    Loricariidae;    oocyte;    reproduction;    semen;   

DOI  :  10.1590/S1516-35982013000100001

来源: SciELO

【 摘 要 】

The objective of this study was to evaluate the effects of water volume and water temperature on the sperm motility duration and the number of spermatozoa, and the water volume on the fertilization rates of oocytes of Rhinelepis aspera. Experiments were carried out to evaluate the effect of semen dilutions (1.74×10-5, 1.74×10-4, 1.74×10-3, 1.74×10-2, 1.74×10-1 and 1.00 mL of sperm.mL-1 of water) and water temperature (5, 10, 15, 20, 25, 30, 35, 40, 45, and 50 ºC) on spermatozoa motility duration. In addition, the effects of insemination dose (7×10³, 7×10(4), 7×10(5), 7×10(6) and 7×10(7) spermatozoa.oocyte-1) and water volume (1.0, 30.0, 60.0, 90.0 and 120.0 mL water.2.0 mL-1 oocytes) on the artificial fertilization rates of oocytes were evaluated. The longest sperm motility duration were observed for the semen dilution of 1.74×10-5 mL semen.mL-1 water and in water at 5 ºC. The highest fertilization rates were obtained for insemination doses between 7.00×10³ and 1.23×10(7) spermatozoa. oocyte-1 and water volume of 28.11 mL water.2.0 mL-1 oocytes.

【 授权许可】

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