Effects of the spermatozoa: oocyte ratio, water volume and water temperature on artificial fertilization and sperm activation of cascudo-preto

Revista Brasileira de Zootecnia

Piana, Pitágoras Augusto³  Sanches, Eduardo Antônio¹  Bombardelli, Robie Allan³  Tessaro, Lucélia¹  Baggio, Diego Mendes³  Sykora, Renata Marino³  Souza, Bruno Estevão de² 

[1] Universidade Estadual Paulista, Jaboticabal, Brazil;Instituto Federal do Paraná, Foz do Iguaçu, Brazil;Universidade Estadual do Oeste do Paraná, Toledo, Brazil

关键词: fish;    Loricariidae;    oocyte;    reproduction;    semen     IntroductionCascudo-preto (Rhinelepis aspera;    Agassiz;    1829) is a species of fish that belongs to the family Loricariidae (Graça & Pavanelli;    2007);   

DOI  :  10.1590/S1516-35982013000100001

学科分类：兽医学

来源: Universidade Federal de Vicosa

【 摘 要 】

The objective of this study was to evaluate the effects of water volume and water temperature on the sperm motility duration and the number of spermatozoa, and the water volume on the fertilization rates of oocytes of Rhinelepis aspera. Experiments were carried out to evaluate the effect of semen dilutions (1.74×10-5, 1.74×10-4, 1.74×10-3, 1.74×10-2, 1.74×10-1 and 1.00 mL of sperm.mL-1 of water) and water temperature (5, 10, 15, 20, 25, 30, 35, 40, 45, and 50 ºC) on spermatozoa motility duration. In addition, the effects of insemination dose (7×103, 7×104, 7×105, 7×106 and 7×107 spermatozoa.oocyte-1) and water volume (1.0, 30.0, 60.0, 90.0 and 120.0 mL water.2.0 mL-1 oocytes) on the artificial fertilization rates of oocytes were evaluated. The longest sperm motility duration were observed for the semen dilution of 1.74×10-5 mL semen.mL-1 water and in water at 5 ºC. The highest fertilization rates were obtained for insemination doses between 7.00×103 and 1.23×107 spermatozoa. oocyte-1 and water volume of 28.11 mL water.2.0 mL-1 oocytes.

【 授权许可】

CC BY-NC