Ki-67 expression in anal intraepithelial neoplasia in AIDS

Sao Paulo Medical Journal

Edenilson Eduardo Calore²  Carmen Ruth Manzione¹  Sidney Roberto Nadal¹  Maria José Cavalieri¹  Nilda Maria Perez Calore²  Regina Paes Dos Santos² 

[1] ,Emílio Ribas Infectology Institute Pathology Section São Paulo,Brazil

关键词: Acquired Immunodeficiency Syndrome;    Condylomata accuminata;    Ki-67;    Anal intraepithelial neoplasia (AIN);    HPV;    Síndrome da Imunodeficiência Adquirida;    Condilomas acuminados Ki-67;    Neoplasia intraepitelial anal (NIA);    HPV;   

DOI  :  10.1590/S1516-31802001000300007

来源: SciELO

【 摘 要 】

CONTEXT: AIDS is one of the most important risk factors for progression and recurrence of anogenital condyloma. In a previous work, we observed that patients with warts and high-grade AIN (HAIN) had recurrences more frequently than did patients with warts without AIN. The mechanisms of this increased incidence of high-grade lesions in AIDS are not known. OBJECTIVE: We studied the expression of the proliferative marker Ki-67 by immunohistochemical methods, in specimens of anal condyloma from HIV+ patients to clarify whether its expression can be associated to the grade of AIN. DESIGN: A retrospective study of hiltological specimens. SETTING: University referral unit. SAMPLE: 34 patients were divided into two groups: (1) condylomas with low grade AIN (LAIN), with 25 patients; and (2) condylomas with HAIN, with 9 patients. In this latter group we examined two areas: 2A (HAIN area) and 2B (LAIN area). MAIN MEASUREMENTS: The immunohistochemical reaction for Ki-67 was done on histological sections. Slices were lightly stained with hematoxylin, to help us in Ki-67 positive cell counting. The percentage of Ki-67 marked nuclei was calculated. We applied one-way variance analysis for statistics. RESULTS: The mean number of Ki-67 positive cells in group 1 was 19.68 ± 10.99; in group 2 (area A) it was 46.73 ± 10.409; and in area B it was 36.43 ± 14.731. There were statistical differences between groups 1 and 2A and between groups 1 and 2B. Ki-67 positive cells predominated in the lower layer in LAIN. Positive Ki-67 cells were found in all layers in group 2A, and in group 2B they predominated in the two lower or in all layers of the epithelium. CONCLUSIONS: Our results suggest that LAIN areas (using routine staining techniques) in HAIN can have a biological behavior more similar to HAIN.

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