Clustering of water bodies in unpolluted and polluted environments based on Escherichia coli phylogroup abundance using a simple interaction database

Genetics and Molecular Biology

Nancy De Castro Stoppe²  Juliana Saragiotto Silva¹  Tatiana Teixeira Torres¹  Camila Carlos²  Elayse Maria Hachich¹  Maria Inês Zanoli Sato¹  Antonio Mauro Saraiva¹  Laura Maria Mariscal Ottoboni² 

[1] ,Universidade Estadual de Campinas Centro de Biologia Molecular e Engenharia Genética Campinas SP ,Brazil

关键词: E. coli;    phylogenetic groups;    pollution sources;    interaction networks;    social network analysis;   

DOI  :  10.1590/S1415-47572014005000016

来源: SciELO

【 摘 要 】

Different types of water bodies, including lakes, streams, and coastal marine waters, are often susceptible to fecal contamination from a range of point and nonpoint sources, and have been evaluated using fecal indicator microorganisms. The most commonly used fecal indicator is Escherichia coli, but traditional cultivation methods do not allow discrimination of the source of pollution. The use of triplex PCR offers an approach that is fast and inexpensive, and here enabled the identification of phylogroups. The phylogenetic distribution of E. coli subgroups isolated from water samples revealed higher frequencies of subgroups A1 and B2(3) in rivers impacted by human pollution sources, while subgroups D1 and D2 were associated with pristine sites, and subgroup B1 with domesticated animal sources, suggesting their use as a first screening for pollution source identification. A simple classification is also proposed based on phylogenetic subgroup distribution using the w-clique metric, enabling differentiation of polluted and unpolluted sites.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License