Construction of a Pig Physical Interactome Using Sequence Homology and a Comprehensive Reference Human Interactome

Evolutionary Bioinformatics

Felix Dreher¹ 

关键词: pig protein-protein interactions;    sequence homology;    bioinformatics;    interaction networks;    genome analysis;   

DOI  :  10.4137/EBO.S8552

学科分类：生物技术

来源: Sage Journals

【 摘 要 】

The analysis of interaction networks is crucial for understanding molecular function and has an essential impact for genome-wide studies. However, the interactomes of most species are largely incomplete and computational strategies that take into account sequence homology can help compensating for this lack of information using cross-species analysis. In this work we report the construction of a porcine interactome resource. We applied sequence homology matching and carried out bi-directional BLASTp searches for the currently available protein sequence collections of human and pig. Using this homology we were able to recover, on average, 71% of the proteins annotated for human pathways for the pig. Porcine protein-protein interactions were deduced from homologous proteins with known interactions in human. The result of this work is a resource comprising 204,699 predicted porcine interactions that can be used in genome analyses in order to enhance functional interpretation of data. The data can be visualized and downloaded from http://cpdb.molgen.mpg.de/pig.

【 授权许可】

Unknown