Chromosomal localization of the 18S-28S and 5S rRNA genes and (TTAGGG)n sequences of butterfly lizards (Leiolepis belliana belliana and Leiolepis boehmei, Agamidae, Squamata)

Genetics and Molecular Biology

Kornsorn Srikulnath²  Yoshinobu Uno¹  Kazumi Matsubara¹  Amara Thongpan¹  Saowanee Suputtitada¹  Somsak Apisitwanich¹  Chizuko Nishida¹  Yoichi Matsuda¹ 

[1] ,Kasetsart University Faculty of Science Department of GeneticsBangkok,Thailand

关键词: chromosomal mapping;    FISH;    interstitial telomeric site;    Leiolepidinae;    ribosomal RNA gene;    telomeric sequence;   

DOI  :  10.1590/S1415-47572011005000042

来源: SciELO

【 摘 要 】

Chromosomal mapping of the butterfly lizards Leiolepis belliana belliana and L. boehmei was done using the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences. The karyotype of L. b. belliana was 2n = 36, whereas that of L. boehmei was 2n = 34. The 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, while the 5S rRNA genes were found in the pericentromeric region of chromosome 6 in both species. Hybridization signals for the (TTAGGG)n sequence were observed at the telomeric ends of all chromosomes, as well as interstitially at the same position as the 18S-28S rRNA genes in L. boehmei. This finding suggests that in L. boehmei telomere-to-telomere fusion probably occurred between chromosome 1 and a microchromosome where the 18S-28S rRNA genes were located or, alternatively, at the secondary constriction of chromosome 1. The absence of telomeric sequence signals in chromosome 1 of L. b. belliana suggested that its chromosomes may have only a few copies of the (TTAGGG)n sequence or that there may have been a gradual loss of the repeat sequences during chromosomal evolution.

【 授权许可】

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