Molecular Cloning and Chromosomal Mapping of Feline p53 Tumor Suppressor Gene

Journal of Veterinary Medical Science

Atsuhiko HASEGAWA¹  Hajime TSUJIMOTO¹  Masaru OKUDA¹  Toshihiro WATARI¹  Yasuyuki MOMOI¹  Yasunobu MATSUMOTO¹  Stephen J. O'BRIEN¹  Akiko UMEDA¹  Ryo GOITSUKA¹ 

关键词: chromosomal mapping;    feline;    molecular cloning;    p53 tumor suppressor gene;   

DOI  :  10.1292/jvms.55.801

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(38)Cited-By(13)Alterations of the p53 tumor suppresser gene have been observed in a variety of human and mouse tumors. For investigation of the role of this gene in tumors in cats, feline p53 cDNA was molecularly cloned by PCR amplifications using primers based on the sequences conserved among several species. The cloned cDNA appeared to cover approximately 90% of the open reading frame of the feline p53 gene and had characteristic structures in common with the p53 genes of several other species. The amino acid sequence similarities of the feline p53 with the human, mouse, rat and chicken counterparts were 82.9%, 75.6%, 76.5% and 57.2% respectively. Moreover, using a panel of feline x rodent somatic cell hybrids, the feline p53 gene was assigned to feline chromosome E1. These data will be useful for determining the role of the p53 tumor suppresser gene in feline tumors.

【 授权许可】

Unknown