Molecular characterization of beta-tubulin from Phakopsora pachyrhizi, the causal agent of Asian soybean rust

Genetics and Molecular Biology

Talles Eduardo Ferreira Maciel²  Maíra Cristina Menezes Freire²  Álvaro M.r. De Almeida¹  Luiz Orlando De Oliveira² 

[1] ,Universidade Federal de Viçosa Departamento de Bioquímica e Biologia Molecular Viçosa MG ,Brazil

关键词: Asian soybean rust;    beta-tubulin;    genetic diversity;    Phakopsora pachyrhizi;    soybeans;   

DOI  :  10.1590/S1415-47572010005000040

来源: SciELO

【 摘 要 】

β-tubulins are structural components of microtubules and the targets of benzimidazole fungicides used to control many diseases of agricultural importance. Intron polymorphisms in the intron-rich genes of these proteins have been used in phylogeographic investigations of phytopathogenic fungi. In this work, we sequenced 2764 nucleotides of the β-tubulin gene (Pp tubB) in samples of Phakopsora pachyrhizi collected from seven soybean fields in Brazil. Pp tubB contained an open reading frame of 1341 nucleotides, including nine exons and eight introns. Exon length varied from 14 to 880 nucleotides, whereas intron length varied from 76 to 102 nucleotides. The presence of only four polymorphic sites limited the usefulness of Pp tubB for phylogeographic studies in P. pachyrhizi. The gene structures of Pp tubB and orthologous β-tubulin genes of Melampsora lini and Uromyces viciae-fabae were highly conserved. The amino acid substitutions in β-tubulin proteins associated with the onset of benzimidazole resistance in model organisms, especially at His6, Glu198 and Phe200, were absent from the predicted sequence of the P. pachyrhizi β-tubulin protein.

【 授权许可】

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