Covering Soybean Leaves With Cellulose Nanofiber Changes Leaf Surface Hydrophobicity and Confers Resistance Against Phakopsora pachyrhizi

Frontiers in Plant Science

Nanami Shiraishi¹  Eiji Yamamura¹  Giyu Usuki¹  Yuji Yamashita¹  Haruka Saito¹  Nanami Sakata¹  Yasuhiro Ishiga¹  Takako Ishiga¹  Viet Tru Nguyen² 

[1] Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan;Western Highlands Agriculture and Forestry Science Institute, Buon Ma Thuot, Vietnam;

关键词: chitin synthase;    Asian soybean rust;    cellulose nanofiber;    hydrophobicity;    Phakopsora pachyrhizi;    pre-infection structure;   

DOI  :  10.3389/fpls.2021.726565

来源: DOAJ

【 摘 要 】

Asian soybean rust (ASR) caused by Phakopsora pachyrhizi, an obligate biotrophic fungal pathogen, is the most devastating soybean production disease worldwide. Currently, timely fungicide application is the only means to control ASR in the field. We investigated cellulose nanofiber (CNF) application on ASR disease management. CNF-treated leaves showed reduced lesion number after P. pachyrhizi inoculation compared to control leaves, indicating that covering soybean leaves with CNF confers P. pachyrhizi resistance. We also demonstrated that formation of P. pachyrhizi appressoria, and also gene expression related to these formations, such as chitin synthases (CHSs), were significantly suppressed in CNF-treated soybean leaves compared to control leaves. Moreover, contact angle measurement revealed that CNF converts soybean leaf surface properties from hydrophobic to hydrophilic. These results suggest that CNF can change soybean leaf surface hydrophobicity, conferring resistance against P. pachyrhizi, based on the reduced expression of CHSs, as well as reduced formation of pre-infection structures. This is the first study to investigate CNF application to control field disease.

【 授权许可】

Unknown